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Experiment K-7-23: Effect of Spaceflight on Level and Function of Immune CellsLymphocytes from the superficial inguinal lymph nodes of rats flown on the Cosmos 2044 space mission were tested for proliferation in response to polyclonal activators. Cells were cultured with T or B cell mitogens, phorbol ester and calcium ionophore, or T cell mitogen and the lymphokines interleukin-1 (IL-1) or interleukin-2 (IL-2), and assayed for DNA synthesis by (3)H-thymidine incorporation. Lymphocytes also were incubated with concanavalin A (Con A), a T cell mitogen, and tested for IL-2 production. Mitogen-stimulated proliferation of lymphocytes from rats exposed to microgravity was not significantly different from synchronous or vivarium controls. Responses to Con A and IL-2, and Con A and IL-1 likewise were unaffected by space flight. Lymphocytes from all of these groups responded well to phorbol ester and calcium ionophore stimulation. Furthermore, lymph node cells (LNC) from control rats and rats flown on Cosmos 2044 produced similar amounts of IL-2. The results obtained using hindlimb suspended rats were notably different from those of flight and control animals. LNC from suspended rats generally had greater proliferative responses to T cell mitogens than did lymphocytes from other groups. Responsiveness to a B cell mitogen was not enhanced. Con A-stimulated LNC from hindlimb suspended rats also produced more IL-2 than did lymphocytes from the other groups. This difference was statistically significant at both IL-2 induction times tested.
Document ID
19960029002
Acquisition Source
Ames Research Center
Document Type
Other
Authors
Nash, P. V.
(Pennsylvania State Univ. University Park, PA United States)
Konstantinova, I. V.
(Institute of Biomedical Problems Moscow, USSR)
Fuchs, B. B.
(Akademiya Meditsinskikh Nauk SSSR Moscow, Russia)
Rakhmilevich, A. L.
(Akademiya Meditsinskikh Nauk SSSR Moscow, Russia)
Lesnyak, A. T.
(Institute of Biomedical Problems Moscow, USSR)
Mastro, A. M.
(Pennsylvania State Univ. University Park, PA United States)
Date Acquired
August 17, 2013
Publication Date
September 1, 1994
Publication Information
Publication: US Experiments Flown on the Soviet Biosatellite Cosmos 2044
Subject Category
Life Sciences (General)
Accession Number
96N29597
Funding Number(s)
CONTRACT_GRANT: NAG2-599
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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