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Prostaglandin E2 Restores Cancellous Bone to Immobilized Limb and Adds Bone to Overloaded Limb in Right Hindlimb Immobilization RatsThe purpose of this study was to determine whether prostaglandin E2 (PGE2) can restore cancellous bone mass and architecture to osteopenic, continuously immobilized (IM), proximal tibial metaphysis (PTM) in female rats. The right hindlimb of three and one-half-month-old Sprague-Dawley female rats were immobilized by right hindlimb immobilization (RHLI) in which the right hindlimb was underloaded and the contralateral left limb was overloaded during ambulation. After 4 or 12 weeks of RHLI, the rats were treated with 3 or 6 mg PGE2/kg/day and RHLI for 8 or 16 weeks. Bone histomorphometry was performed on microradiographs of PTM. Immobilization (IM) induced a transient cancellous bone loss and decreased trabecular thickness, number and node density, and increased free end density that established a new steady state after 4 weeks of IM. Three or 6 mg PGE2/kg/d for 8 weeks beginning at 4 or 12 weeks of IM completely restored cancellous bone mass (+127 to +188 percent) and structure to the age-related control levels in spite of continuous IM. Another 8 weeks of treatment maintained bone mass and architecture at these levels. No differences in cancellous bone mass and architecture were found between the overloaded PTM or RHLI rats and the age-related controls. However, 3 and 6 mg/kg/d of PGE2 treatment started at 4 or 12 weeks for 8 weeks significantly increased cancellous bone mass in the overloaded PTM (+45 to +74% of untreated controls), and another 8 weeks of treatment maintained bone mass at these levels. Our findings indicate that daily 3 or 6 mg PGE2/kg/d treatment restores and maintains PTM cancellous bone mass in continuously immobilized (right) tibiae, and adds and maintains extra bone to slightly overloaded PTM cancellous bone in female rats.
Document ID
19970004459
Acquisition Source
Ames Research Center
Document Type
Reprint (Version printed in journal)
Authors
Li, M.
(Utah Univ. Salt Lake City, UT United States)
Jee, W. S. S.
(Utah Univ. Salt Lake City, UT United States)
Ke, H. Z.
(Utah Univ. Salt Lake City, UT United States)
Liang, X. G.
(Utah Univ. Salt Lake City, UT United States)
Lin, B. Y.
(Utah Univ. Salt Lake City, UT United States)
Ma, Y. F.
(Utah Univ. Salt Lake City, UT United States)
Setterberg, R. B.
(Utah Univ. Salt Lake City, UT United States)
Date Acquired
August 17, 2013
Publication Date
January 1, 1993
Publication Information
Publication: Bone
Publisher: Pergamon Press, Ltd.
Volume: 14
ISSN: 8756-3282
Subject Category
Life Sciences (General)
Report/Patent Number
NASA-CR-202477
NAS 1.26:202477
Accession Number
97N70324
Funding Number(s)
CONTRACT_GRANT: DE-AC02-76EV-001119
CONTRACT_GRANT: DE-FG02-89ER-60764
CONTRACT_GRANT: AR-38346
CONTRACT_GRANT: NAG2-435
Distribution Limits
Public
Copyright
Public Use Permitted.
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