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Molecular Basis of the Increase in Invertase Activity Elicited by Gravistimulation of Oat-Shoot PulviniAn asymmetric (top vs. bottom) increase in invertase activity is elicited by gravistimulation in oatshoot pulvini starting within 3h after treatment. In order to analyze the regulation of invertase gene expression in this system, we examined the effect of gravistimulation on invertase mRNA induction. Total RNA and poly(A)(+)RNA, isolated from oat pulvini, and two oligonucleotide primers, corresponding to two conserved amino-acid sequences (NDPNG and WECPD) found in invertase from other species, were used for the Polymerase Chain Reaction (PCR). A partial-length cDNA (550 base pairs) was obtained and characterized. There was a 52 % deduced amino-acid sequence homology to that of carrot beta-fructosi- dase and a 48 % homology to that of tomato invertase. Northern blot analysis showed that there was an obvious transient accumulation of invertase mRNA elicited by gravistimulation of oat pulvini. The mRNA was rapidly induced to a maximum level at 1h following gravistimulation treatment and gradually decreased afterwards. The mRNA level in the bottom half of the oat pulvinus was significantly higher (five-fold) than that in the top half of the pulvinus tissue. The induction of invertase mRNA was consistent with the transient enhancement of invertase activity during the graviresponse of the pulvinus. These data indicate that the expression of the invertase gene(s) could be regulated by gravistimulation at the transcriptional and/or translational levels. Southern blot analysis showed that there were four genomic DNA fragments hybridized to the invertase cDNA. This suggests that an invertase gene family may exist in oat plants.
Document ID
19970017422
Acquisition Source
Kennedy Space Center
Document Type
Reprint (Version printed in journal)
Authors
Wu, Liu-Lai
(Michigan Univ. Ann Arbor, MI United States)
Song, Il
(Michigan Univ. Ann Arbor, MI United States)
Kim, Donghern
(Michigan Univ. Ann Arbor, MI United States)
Kaufman, Peter B.
(Michigan Univ. Ann Arbor, MI United States)
Date Acquired
August 17, 2013
Publication Date
January 15, 1993
Publication Information
Publication: J. Plant Physiol.
Publisher: Gustav Fischer Verlag
Volume: 142
Subject Category
Life Sciences (General)
Report/Patent Number
NASA-CR-203751
NAS 1.26:203751
Accession Number
97N71633
Funding Number(s)
CONTRACT_GRANT: NAGw-1600
CONTRACT_GRANT: NAG10-0069
Distribution Limits
Public
Copyright
Public Use Permitted.
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