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Studies for Somatic Embryogenesis in Sweet PotatoThe purpose of this study was to improve the somatic embryo (SE) system for plant production of sweet potato (Ipomoea batatas L(Lam)). Explants isolated from SE-derived sweet potato plants were compared with control (non SE-derived) plants for their competency for SE production. Leaf explants were cultured on Murashige-Skoog (MS) medium with 2,4-dichlorophenoxy acetic acid (0.2 mg/L) and 6-benzylaminopurine (2.5 mg/L) for 2 weeks in darkness and transferred to MS medium with abscisic acid (2.5 mg/L). Explants isolated from those plants developed through somatic embryogenesis produced new somatic embryos rapidly and in higher frequency than those isolated from control plants They also appeared to grow faster in tissue culture than the control plants. Current studies in the laboratory are examining whether plants derived from a cyclical embryogenesis system (five cycles) would have any further positive impact on the rapidity and frequency of somatic embryo development. More detailed studies using electron microscopy are expected to show the point of origin of the embryos and to allow determination of their quality throughout the cyclical process. This study may facilitate improved plant micropropagation, gene transfer and germplasm conservation in sweet potato.
Document ID
19970028668
Document Type
Conference Paper
Authors
Bennett, J. Rasheed (Tuskegee Inst. AL United States)
Prakash, C. S. (Tuskegee Inst. AL United States)
Date Acquired
August 17, 2013
Publication Date
January 1, 1997
Publication Information
Publication: The First National Student Conference: NASA University Research Centers at Minority Institutions
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGw-2940
Distribution Limits
Public
Copyright
Public Use Permitted.
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