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Record Details

Record 87 of 9456
Endothelial Cell Morphology and Migration are Altered by Changes in Gravitational Fields
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Author and Affiliation:
Melhado, Caroline(Morehouse School of Medicine, Dept. of Biochemistry, Atlanta, GA United States)
Sanford, Gary(Morehouse School of Medicine, Dept. of Biochemistry, Atlanta, GA United States)
Harris-Hooker, Sandra(Morehouse School of Medicine, Dept. of Biochemistry, Atlanta, GA United States)
Abstract: Endothelial cell migration is important to vascular wall regeneration following injury or stress. However, the mechanism(s) governing this response is not well understood. The microgravity environment of space may complicate the response of these cells to injury. To date, there are no reports in this area. We examined how bovine aortic (BAEC) and pulmonary (BPEC) endothelial cells respond to denudation injury under hypergravity (HGrav) and simulated microgravity (MGrav), using image analysis. In 10% FBS, the migration of confluent BAEC and BPEC into the denuded area was not affected by HGrav or MGrav. However, in low FBS (0.5%), signficantly retarded migration under MGrav, and increased migration under HGrav was found. MGrav also decreased the migration of postconfluent BPEC while HGrav showed no difference. Both MGrav and HGrav strongly decreased the migration of postconfluent BAEC. Also, both cell lines showed significant morphological changes by scanning electron microscopy. These studies indicate that endothelial cell function is affected by changes in gravity.
Publication Date: Jan 01, 1997
Document ID:
19970028670
(Acquired Oct 16, 1997)
Accession Number: 97N27399
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Conference Paper
Publication Information: The First National Student Conference: NASA University Research Centers at Minority Institutions; 100-103; (NASA-CR-205049); (SEE 19970028662)
Contract/Grant/Task Num: NAG9-644; NIH-RR-03034
Financial Sponsor: NASA Johnson Space Center; Houston, TX United States
National Inst. of Health; Bethesda, MD United States
Organization Source: Morehouse School of Medicine; Dept. of Biochemistry; Atlanta, GA United States
Description: 4p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright; Distribution as joint owner in the copyright
NASA Terms: GRAVITATIONAL FIELDS; CELLS (BIOLOGY); MICROGRAVITY; MORPHOLOGY; MIGRATION; INJURIES; IMAGE ANALYSIS; HIGH GRAVITY ENVIRONMENTS; CARDIOVASCULAR SYSTEM; SCANNING ELECTRON MICROSCOPY; CYTOLOGY
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