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Solubilization and Resolution of the Membrane-Bound Nitrite Reductase from Paracoccus Halodenitrificans into Nitrite and Nitric Oxide ReductasesMembranes prepared from Paracoccus halodenitrificans reduced nitrite or nitric oxide to nitrous oxide. Extraction of these membranes with the detergent CHAPSO [3-(3-Chlolamidoporopyldimethylammonio)-1-(2- hydroxy-1-propanesulfonate)], followed by ammonium sulfate fractionation of the solubilized proteins, resulted in the separation of nitrite and nitric oxide reductase activities. The fraction containing nitrite reductase activity spectrally resembled a cd-type cytochrome. Several cytochromes were detected in the nitric oxide reductase fraction. Which, if any, of these cytochromes is associated with the reduction of nitric oxide is not clear at this time.
Document ID
19990019283
Acquisition Source
Ames Research Center
Document Type
Other
External Source(s)
Authors
Grant, Michael A.
(Santa Clara Univ. CA United States)
Cronin, Sonja E.
(NASA Ames Research Center Moffett Field, CA United States)
Hochstein, Lawrence I.
(NASA Ames Research Center Moffett Field, CA United States)
Date Acquired
August 19, 2013
Publication Date
January 1, 1984
Publication Information
Publication: Archives of Microbiology
Publisher: Springer-Verlag
Volume: 140
Subject Category
Chemistry And Materials (General)
Funding Number(s)
CONTRACT_GRANT: NCA2-OR-685-203
Distribution Limits
Public
Copyright
Other

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