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The Effects of Orbital Spaceflight on Human Osteoblastic Cell Physiology and Gene ExpressionThe purpose of the proposed study is to establish whether changes in gravitational loading have a direct effect on osteoblasts to regulate TGF-6 expression. The effects of spaceflight and reloading on TGF-B MRNA and peptide levels will be studied in a newly developed line of immortalized human fetal osteoblasts (HFOB) transfected with an SV-40 temperature dependent mutant to generate proliferating, undifferentiated hFOB cells at 33-34 C and a non-proliferating, differentiated HFOB cells at 37-39'C. Unlike previous cell culture models, HFOB cells have unlimited proliferative capacity yet can be precisely regulated to differentiate into mature cells which express mature osteoblast function. If isolated osteoblasts respond to changes in mechanical loading in a manner similar to their response in animals, the cell system could provide a powerful model to investigate the signal transduction pathway for gravitational loading.
Document ID
20000005005
Acquisition Source
Ames Research Center
Document Type
Other
Authors
Turner, R. T.
(NASA Ames Research Center Moffett Field, CA United States)
Date Acquired
September 7, 2013
Publication Date
January 1, 1999
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: NAG2-896
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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