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differences in early t-cell signaling in cultures grown in a rotating clinostat vs. static controlsAltered gravity has previously been demonstrated to be a stress that can influence components of the immune system. Specifically, T-cell activation has been shown to be affected by changes in gravity, exhibiting a decrease in proliferative response to in vitro stimulation in microgravity. Subsequent ground based studies utilizing a rotating clinostat to model some of the effects of microgravity have been consistent with earlier flight based experiments. These ground and flight experiments have examined T-cell activation by measuring various responses including production of cytokines, DNA synthesis and the production of various cell surface activation markers. These indicators of T-cell activation were measured anywhere from 4 to 72 hours after stimulation. Prior to the work described here, the initial signaling events in T-cell activation had not been directly examined. The goal of this project was to determine how the process of early signal transduction was affected by growth in a rotating clinostat. Here we directly show a defect in signaling from TCR to MAPK in purified peripheral T-cells activated in the clinostat by OKT3/antiCD28 coated microbeads as compared to static controls.
Document ID
20000110098
Document Type
Conference Paper
Authors
Alexamder. M.
(NASA Johnson Space Center Houston, TX United States)
Nelman-Gonzales, M.
(NASA Johnson Space Center Houston, TX United States)
Penkala, J.
(NASA Johnson Space Center Houston, TX United States)
Sams, C.
(NASA Johnson Space Center Houston, TX United States)
Date Acquired
August 19, 2013
Publication Date
January 1, 1999
Subject Category
Life Sciences (General)
Meeting Information
28th Annual Automn Immunology Conference(Chicago, IL)
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.