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Quantifying Main Trends in Lysozyme Nucleation: The Effect of Precipitant Concentration and Impurities
Author and Affiliation:
Burke, Michael W.(Alliance for Microgravity Materials Science and Applications, Huntsville, AL United States)
Judge, Russell A.(Alliance for Microgravity Materials Science and Applications, Huntsville, AL United States)
Pusey, Marc L.(NASA Marshall Space Flight Center, Huntsville, AL United States)
Rose, M. Franklin [Technical Monitor]
Abstract: Full factorial experiment design incorporating multi-linear regression analysis of the experimental data allows the main trends and effects to be quickly identified while using only a limited number of experiments. These techniques were used to identify the effect of precipitant concentration and the presence of an impurity, the physiological lysozyme dimer, on the nucleation rate and crystal dimensions of the tetragonal form of chicken egg white lysozyme. Increasing precipitant concentration was found to decrease crystal numbers, the magnitude of this effect also depending on the supersaturation. The presence of the dimer generally increased nucleation. The crystal axial ratio decreased with increasing precipitant concentration independent of impurity.
Publication Date: Jan 01, 2000
Document ID:
20000110583
(Acquired Nov 17, 2000)
Subject Category: SOLID-STATE PHYSICS
Document Type: Preprint
Contract/Grant/Task Num: NCC8-66
Financial Sponsor: NASA Marshall Space Flight Center; Huntsville, AL United States
Organization Source: NASA Marshall Space Flight Center; Huntsville, AL United States
Description: 2p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: No Copyright
NASA Terms: IMPURITIES; LYSOZYME; NUCLEATION; CONCENTRATION (COMPOSITION); DIMERS; PRECIPITATES; CHICKENS; CRYSTALS; REGRESSION ANALYSIS; SUPERSATURATION; FACTORIAL DESIGN; EGGS
Availability Source: Other Sources
Availability Notes: Abstract Only
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