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Comparative Study in Laboratory Rats to Validate Sperm Quality Methods and EndpointsAbstract The Naval Health Research Center, Detachment (Toxicology) performs toxicity studies in laboratory animals to characterize the risk of exposure to chemicals of Navy interest. Research was conducted at the Toxicology Detachment at WPAFB, OH in collaboration with Wright State University, Department of Biological Sciences for the validation of new bioassay methods for evaluating reproductive toxicity. The Hamilton Thorne sperm analyzer was used to evaluate sperm damage produced by exposure to a known testicular toxic agent, methoxyacetic acid and by inhalation exposure to JP-8 and JP-5 in laboratory rats. Sperm quality parameters were evaluated (sperm concentration, motility, and morphology) to provide evidence of sperm damage. The Hamilton Thorne sperm analyzer utilizes a DNA specific fluorescent stain (similar to flow cytometry) and digitized optical computer analysis to detect sperm cell damage. The computer assisted sperm analysis (CASA) is a more rapid, robust, predictive and sensitive method for characterizing reproductive toxicity. The results presented in this poster report validation information showing exposure to methoxyacetic acid causes reproductive toxicity and inhalation exposure to JP-8 and JP-5 had no significant effects. The CASA method detects early changes that result in reproductive deficits and these data will be used in a continuing program to characterize the toxicity of chemicals, and combinations of chemicals, of military interest to formulate permissible exposure limits.
Document ID
20010002149
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Price, W. A.
(Geo-Centers, Inc. Wright-Patterson AFB, OH United States)
Briggs, G. B.
(Geo-Centers, Inc. Wright-Patterson AFB, OH United States)
Alexander, W. K.
(Naval Health Research Center Wright-Patterson AFB, OH United States)
Still, K. R.
(Naval Health Research Center Wright-Patterson AFB, OH United States)
Grasman, K. A.
(Wright State Univ. Dayton, OH United States)
Date Acquired
August 20, 2013
Publication Date
May 1, 2000
Publication Information
Publication: JANNAF 18th Safety and Environmental Protection Subcommittee Meeting
Subject Category
Life Sciences (General)
Meeting Information
Meeting: Safety and Environmental Protection
Location: Cocoa Beach, FL
Country: United States
Start Date: May 8, 2000
End Date: May 12, 2000
Sponsors: Department of the Navy, Department of the Air Force, NASA Headquarters, Department of the Army
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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