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The Effects of Long-Term Feeding of Rodent Food Bars on Lipid Peroxidation And Antioxidant Enzyme Levels In Fisher RatsA specialized rodent food bar diet has been developed and utilized successfully for short-duration shuttle missions. Recent tests conducted in preparation for experiments aboard the International Space Station (ISS) indicated that long-term food bar feeding for three months induced hyperlipidemia in rats. This study examined oxidative stress status in livers of these same animals. Spectrophotometric analysis of 79 Fischer rat livers (40 female and 39 male) for lipid peroxidation (LPO) and superoxide dismutase (SOD) was conducted using Bioxytech LPO-587(TM) assay kit and SOD-525(Tm) assay kit, respectively. The treatment groups consisted of 20 male CHOW and 19 male FOOD BAR rats and 20 female CHOW and 20 female FOOD BAR rats. Statistical analysis to compare differences between groups was performed by standard analysis of variance procedures. The male FOOD BAR group LPO mean (3.6 +/- 0.2 mmol/g) was significantly (p less than or equal to 0.05) greater than that of the male CHOW group (2.1 +/-0.1 mmol/g). Moreover the female FOOD BAR group LPO mean (2.9 +/-0.1 mmol/g) was also significantly greater than the female CHOW group mean (2.2 +/-0.1 mmol/g). The mean values for SOD in both male and female groups showed no significant differences between CHOW and FOOD BAR groups. These results show that LPO levels were significantly higher in both the male and female FOOD BAR groups compared to CHOW groups and that there was no concomitant increase in SOD levels across the group. In addition, males showed a greater difference than females in terms of LPO levels. These findings suggest a need for further investigation into the use of the current food bar formulation for long-term experiments such as those planned for the ISS.
Document ID
20010119528
Acquisition Source
Ames Research Center
Document Type
Other
Authors
Ramirez, Joel
(Lockheed Martin Corp. Moffett Field, CA United States)
Zirkle-Yoshida, M.
(Foothill-De Anza Community Coll. Los Altos Hills, CA United States)
Piert, S.
(Lockheed Martin Corp. Moffett Field, CA United States)
Barrett, J.
(Adecco Moffett Field, CA United States)
Yul, D.
(Lockheed Martin Corp. Moffett Field, CA United States)
Dalton, B.
(NASA Ames Research Center Moffett Field, CA United States)
Girten, B.
(NASA Ames Research Center Moffett Field, CA United States)
Date Acquired
August 20, 2013
Publication Date
January 1, 2001
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAS2-14263
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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