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Record 46 of 8620
Carbon Isotope Fractionations Associated with Methanotrophic Growth with the Soluble and Particulate Methane Monooxygenases
Author and Affiliation:
Jahnke, Linda L.
Summons, Roger E.
Chang, Sherwood [Technical Monitor]
Abstract: Growth experiments with the RuMP-type methanotroph, Methylococcus capsulatus (Bath), have demonstrated that biomass and lipid biomarkers are significantly depleted in C-13 compared to the substrate methane and that the extent of fractionation is dependent on whether cells express the soluble (s) or particulate (p) methane monooxygenase (MMO). The presence or absence of the characteristic sMMO subunits was monitored using SDS-polyacrylamide gels. In M. capsulatus grown with no Cu supplementation, the characteristic sMMO subunits were observed in the soluble fraction throughout the entire growth period and biomass was depleted in C-13 by approximately 14,700 relative to substrate methane. In cells grown with 5uM Cu, no sMMO bands were observed and a greater fractionation of approximately 27,700 in resultant biomass was obtained. Methanol growth experiments with M. capsulatus and with a RuMP methylotroph, Methylophilus methylotrophus, in which biomass measurements yielded depletions in C-13 of 9 and 5%(sub o), respectively, suggest that oxidation of methane is the major fractionation step. Growth of M. capsulatus at a low level of oxygen, approximately 0.5%, had no significant effect on carbon isotope fractionation by either sMMO or pMMO. These observations are significant for identification of molecular biomarkers; and methanotrophic contributions to carbon isotope composition in natural environments.
Publication Date: Jan 01, 1996
Document ID:
20020032684
(Acquired Mar 29, 2002)
Subject Category: INORGANIC, ORGANIC AND PHYSICAL CHEMISTRY
Document Type: Preprint
Meeting Information: 96th American Chemical Society Meeting; 20-23 May 1996; New Orleans, LA; United States
Meeting Sponsor: American Chemical Society; United States
Contract/Grant/Task Num: RTOP 185-52-32
Financial Sponsor: NASA Ames Research Center; Moffett Field, CA United States
Organization Source: NASA Ames Research Center; Moffett Field, CA United States
Australian Geological Survey Organisation, Canberra; Australian Capital Territory, Australia
Description: 1p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: No Copyright
NASA Terms: CARBON ISOTOPES; FRACTIONATION; METHANE; PARTICULATES; METHYL ALCOHOL; BIOMASS; CARBON 13; SUBSTRATES
Availability Source: Other Sources
Availability Notes: Abstract Only
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