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From Field to Laboratory: A New Database Approach for Linking Microbial Field Ecology with Laboratory StudiesThe Ames Exobiology Culture Collection Database (AECC-DB) has been developed as a collaboration between microbial ecologists and information technology specialists. It allows for extensive web-based archiving of information regarding field samples to document microbial co-habitation of specific ecosystem micro-environments. Documentation and archiving continues as pure cultures are isolated, metabolic properties determined, and DNA extracted and sequenced. In this way metabolic properties and molecular sequences are clearly linked back to specific isolates and the location of those microbes in the ecosystem of origin. Use of this database system presents a significant advancement over traditional bookkeeping wherein there is generally little or no information regarding the environments from which microorganisms were isolated. Generally there is only a general ecosystem designation (i.e., hot-spring). However within each of these there are a myriad of microenvironments with very different properties and determining exactly where (which microenvironment) a given microbe comes from is critical in designing appropriate isolation media and interpreting physiological properties. We are currently using the database to aid in the isolation of a large number of cyanobacterial species and will present results by PI's and students demonstrating the utility of this new approach.
Document ID
20020051386
Acquisition Source
Ames Research Center
Document Type
Conference Paper
Authors
Bebout, Leslie
(NASA Ames Research Center Moffett Field, CA United States)
Keller, R.
(NASA Ames Research Center Moffett Field, CA United States)
Miller, S.
(North Carolina State Univ. Raleigh, NC United States)
Jahnke, L.
(NASA Ames Research Center Moffett Field, CA United States)
DeVincenzi, D.
Date Acquired
August 20, 2013
Publication Date
January 1, 2002
Subject Category
Life Sciences (General)
Meeting Information
Meeting: Astrobiology Science Conference
Country: Unknown
Start Date: April 1, 2002
Funding Number(s)
PROJECT: RTOP 100-20-10
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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