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Determining the Absorbance Spectra of Photochromic Materials From Measured Spectrophotometer DataIf a two-state photochromic material is optically bleached, the absorbance spectrum data measured by a spectrophotometer is in general comprised of components from both the ground state and the upper state. Under general conditions, it may be difficult to extract the actual upper state spectrum from the spectrum of the bleached material. A simple algorithm is presented here for the recovery of the pure absorbance spectra of the upper state of a material such as bacteriorhodopsin, given single wavelength bleaching illumination, steady-state conditions, and accurate knowledge of phototransition rates and thermal decay rates.
Document ID
20020054269
Document Type
Preprint (Draft being sent to journal)
Authors
Downie, John D. (NASA Ames Research Center Moffett Field, CA United States)
Date Acquired
August 20, 2013
Publication Date
January 1, 1998
Subject Category
Optics
Funding Number(s)
PROJECT: RTOP 632-10-13
Distribution Limits
Public
Copyright
Other