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Desulfonatronum paiuteum sp. nov.: A New Alkaliphilic, Sulfate-Reducing Bacterium, Isolated from Soda Mono Lake, CaliforniaA novel alkaliphilic, sulfate reducing bacterium strain MLF1(sup T) was isolated from sediments of soda Mono Lake, California. Gram-negative vibrion cells, motile by singular polar flagellum, with sizes 0.5 - 0.6x 1.2 - 2.0 micron occurred singly, in pairs or short spirilla. Growth was observed over the temperature range of +15 C to +48 C (optimum +37 C), NaCl concentration range is greater than 1 - 7 %, wt/vol (optimum 3 %, wt/vol) and pH range 7.8 - 10.5 (optimum pH 9.0 - 9.4). The novel isolate is strictly alkaliphilic, requires high carbonate concentration in medium, obligately anaerobic and catalase negative. As electron donors strain MLF1(sup T) uses hydrogen, formate, ethanol. Sulfate, sulfite, and thiosulfate (but not sulfur or nitrate) can be used as electron acceptors. The sole end product of growth on formate was H2S. Strain MLF1(sup T) is resistant to kanamycin and gentamycin, but sensitive to chloramphenicol and tetracycline. Na2MoO4 inhibits growth of strain MLF1(sup T). The sum of G+C in DNA is 63.1 mol% (by HPLC method). On the basis of physiological and molecular properties, the isolate was considered as novel species of genus Desulfonatronum; and the name Desulfonatronum paiuteum sp. nov., is proposed (type strain MLF1(sup T) = ATCC BAA-395(sup T) = DSMZ 14708(sup T).
Document ID
Acquisition Source
Marshall Space Flight Center
Document Type
Pikuta, Elena
(Alabama Univ. Huntsville, AL United States)
Hoover, Richard B.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Marsic, Damien
(Alabama Univ. Huntsville, AL United States)
Whitman, William
(Georgia Univ. Athens, GA United States)
Cleland, David
(American Type Culture Collection Manassas, VA United States)
Krader, Paul
(American Type Culture Collection Manassas, VA United States)
Six, N. Frank
Date Acquired
August 20, 2013
Publication Date
January 1, 2002
Subject Category
Life Sciences (General)
Distribution Limits
Work of the US Gov. Public Use Permitted.

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