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Spirochaeta americana sp. nov.: A New Haloalkaliphilic, Obligately Anaerobic Spirochete Isolated from Soda Mono Lake, CaliforniaA novel obligately anaerobic, mesophilic, haloalkaliphilic spirochete, strain ASpG1, was isolated from sediments of the alkaline, hypersaline Mono Lake in California, U.S.A. The gram-negative cells are motile and spirochete-shaped with sizes of 0.22 x 10-15 micron. Growth was observed over the temperature range of 10 C to 44 C (optimum 37 C), NaCl concentration range of greater than 1 - 12 % (wt/vol) (optimum 3%), and pH range 7.5 - 10.5 (optimum pH 9.5). The novel isolate is strictly alkaliphilic, requires high concentrations of carbonate in the medium, and is capable of utilizing D-glucose, fructose, maltose, sucrose, starch, and D-mannitol. Main end products of glucose fermentation are: H2, acetate, ethanol, and formate. Strain AspG1 is resistant to kanamycin, but sensitive to chloramphenicol, gentamycin and tetracycline. The G+C content of its DNA is 58.5 mol%. On the basis of its physiological and molecular properties, the isolate appears to be a novel species among the genus Spirochaeta; and the name Spirochaeta americana sp. nov., is proposed for the taxon (type strain ASpG1(sup T) = ATCC BAA_392(sup T) = DSMZ 14872(sup T)).
Document ID
Acquisition Source
Marshall Space Flight Center
Document Type
Hoover, Richard B.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Pikuta, Elena V.
(Alabama Univ. Huntsville, AL United States)
Marsic, Damien
(Alabama Univ. Huntsville, AL United States)
Whitman, William B.
(Georgia Univ. Athens, GA United States)
Tang, Jane
(American Type Culture Collection Manassas, VA United States)
Krader, Paul
(American Type Culture Collection Manassas, VA United States)
Six, N. Frank
Date Acquired
August 20, 2013
Publication Date
January 1, 2002
Subject Category
Life Sciences (General)
Distribution Limits
Work of the US Gov. Public Use Permitted.

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