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Equilibrium Kinetics Studies and Crystallization Aboard the International Space Station (ISS) Using the Protein Crystallization Apparatus for Microgravity (PCAM)Protein Crystallization Apparatus in Microgravity (PCAM) trays have been used in Shuttle missions to crystallize proteins in a microgravity environment. The crystallization experiments are 'sitting drops' similar to that in Cryschem trays, but the reservoir solution is soaked in a wick. From early 2001, crystallization experiments are conducted on the International Space Station using mission durations of months rather than two weeks on previous shuttle missions. Experiments were set up in April 2001 on Flight 6A to characterize the time crystallization experiments will take to reach equilibrium in a microgravity environment using salts, polyethylene glycols and an organic solvent as precipitants. The experiments were set up to gather data for a series of days of activation with different droplet volumes and precipitants. The experimental set up on ISS and results of this study will be presented. These results will help future users of PCAM to choose precipitants to optimize crystallization conditions for their target macromolecules for a particular mission with known mission duration. Changes in crystal morphology and size between the ground and space grown crystals of a protein and a protein -DNA complex flown on the same mission will also be presented.
Document ID
Document Type
Conference Paper
Achari, Aniruddha
(Raytheon Co. Huntsville, AL United States)
Roeber, Dana F.
(Universities Space Research Association Huntsville, AL United States)
Barnes, Cindy L.
(Universities Space Research Association Huntsville, AL United States)
Kundrot, Craig E.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Stinson, Thomas N.
Date Acquired
August 20, 2013
Publication Date
January 1, 2002
Subject Category
Space Processing
Meeting Information
Meeting: American Crystallographic Association Annual Meeting
Location: San Antonio, TX
Country: United States
Start Date: May 25, 2002
End Date: May 30, 2002
Sponsors: American Crystallographic Association
Distribution Limits
Work of the US Gov. Public Use Permitted.

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