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Effects of Microgravity On Oxidative and Antioxidant Enzymes In Mouse Hindlimb MuscleGastrocnemius muscle of mice were analyzed in order to examine the effects of 12 days of microgravity on the oxidative enzyme climate synthase (CS) and the antioxidant enzyme superoxide dismutase (SOD). The female C57BL/6J mice utilized for this study were part of the Commercial Biomedical Testing Module (CBTM) payload that flew aboard STS-108. Mice were housed in Animal Enclosure Modules (AEMs) provided by NASA Ames. The flight (FLT) group and the ground control (CON) group each had 12 mice per group. The AEMs that held the CON group operated on a 48-hour delay from the FLT group and were located inside the Orbital Environmental Simulator (OES) at Kennedy Space Center. The temperature, CO2 and relative humidity inside the OES was regulated based on downlinked information from the shuttle middeck. Student T tests were used to compare groups and a p < 0.05 was used to determine statistical significance. Results indicated that CS levels for the FLT group were significantly lower than the CON group while the SOD levels were significantly higher. The CS FLT mean was 19% lower and the SOD FLT mean was 17% higher than the respective CON group means. Although these findings are among the first muscle enzyme values reported for mice from a shuttle mission, these results are similar to some results previously reported for rats exposed to microgravity or hindlimb suspension. The changes seen during the CBTM payload are reflective of the deconditioning that takes place with disuse of the hindlimbs and indicate that muscle enzyme changes induced by disuse deconditioning are similar in both rodent species.
Document ID
20020074613
Acquisition Source
Ames Research Center
Document Type
Conference Paper
Authors
Girten, B.
(NASA Ames Research Center Moffett Field, CA United States)
Hoopes, R.
(Lockheed Martin Corp. Moffett Field, CA United States)
Steele, M.
(Lockheed Martin Corp. Moffett Field, CA United States)
Morony, S.
(Amgen Pharmaceticals CA United States)
Bateman, T. A.
(BioServe Space Technologies Boulder, CO United States)
Sun, S.
Date Acquired
August 20, 2013
Publication Date
January 1, 2002
Subject Category
Life Sciences (General)
Meeting Information
Meeting: 18th ASGSB Annual Meeting Issue
Location: Cape Canaveral, FL
Country: United States
Start Date: November 6, 2002
End Date: November 9, 2002
Sponsors: American Society for Gravitational and Space Biology
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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