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Cell and Molecular Biology of Ataxia Telangiectasia Heterozygous Human Mammary Epithelial Cells Irradiated in CultureAutologous isolates of cell types from obligate heterozygotes with the autosomal disorder ataxia-telangiectasia (A-T)were used to begin a tissue culture model for assessing pathways of radiation-induced cancer formation in this target tissue. This was done by establishing cultures of stromal fibroblasts and long-term growth human mammary epithelial cells (HMEC) in standard 2-dimensional tissue culture in order to establish expression of markers detailing early steps of carcinogenesis. The presumptive breast cancer susceptibility of A-T heterozygotes as a sequel to damage caused by ionizing radiation provided reason to study expression of markers in irradiated HMEC. Findings from our study with HMEC have included determination of differences in specific protein expression amongst growth phase (e.g., log vs stationary) and growth progression (e.g., pass 7 vs pass 9), as well as differences in morphologic markers within populations of irradiated HMEC (e.g., development of multinucleated cells).
Document ID
20020074847
Document Type
Contractor or Grantee Report
Authors
Richmond, Robert C.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Date Acquired
September 7, 2013
Publication Date
September 1, 2001
Subject Category
Life Sciences (General)
Report/Patent Number
AD-A403375
Funding Number(s)
CONTRACT_GRANT: DAMD17-99-IA-9382
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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