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Record 21 of 878
Spirochaeta Americana Sp. Nov., A new Haloalkaliphilic, Obligately Anaerobic Spirochete Isolated from Soda Mono Lake in California
Author and Affiliation:
Hoover, Richard B.(NASA Marshall Space Flight Center, Huntsville, AL United States)
Pikuta, Elena V.(Alabama Univ., Center for Space Plasma and Aeronautic Research, Huntsville, AL United States)
Bej, Asim K.(Alabama Univ., Dept. of Biology, Birmingham, AL United States)
Marsic, Damien(Alabama Univ., Lab. for Structural Biology, Huntsville, AL United States)
Whitman, William B.(Georgia Univ., Dept. of Microbiology, Athens, GA United States)
Tang, Jane(American Type Culture Collection, Manassas, VA United States)
Krader, Paul(American Type Culture Collection, Manassas, VA United States)
Six, N. Frank [Technical Monitor]
Abstract: A novel obligately anaerobic, mesophilic, haloalkaliphilic spirochete, strain ASpG1(sup T), was isolated from sediments of the alkaline, hypersaline Mono Lake in California, U.S.A. The Gram-negative cells are motile and spirochete-shaped with sizes of 0.2 - 0.22 X 8-15 microns. Growth was observed over the following ranges: temperature 10 C to 44 C; optimum +37 C; NaCl concentration 2 - 12 % (w/v); optimum NaCl3 % and pH 8 - 10.5; optimum pH 9.5. The novel isolate is strictly alkaliphilic, requires high concentrations of carbonate in the medium, and is capable of utilizing D-glucose, fructose, maltose, sucrose, starch, and D-mannitol. The main end products of glucose fermentation are: H2, acetate, ethanol, and formate. Strain ASpG(sup T) is resistant to kanamycin, and rifampin, but sensitive to chloramphenicol, gentamycin and tetracycline. The G+C content of its DNA is 58.5 mol%, genome size is 2.98 x l0(exp 9) Daltons, Tm of the genomic DNA is 68 +/- 2 C, and DNA-DNA hybridization with the most closely related species, Spirocheta alkalica Strain Z-7491(sup T), exhibited 48.7% homology. On the basis of its physiological and molecular properties, the isolate appears to be a novel species of the genus Spirochaeta; and the name Spirochaeta americana sp. nov., is proposed for the taxon (type strain ASpG1(sup T) = ATCC BAA-392(sup T) = DSMZ 14872(sup T)).
Publication Date: Jan 01, 2002
Document ID:
(Acquired Jan 03, 2003)
Document Type: Preprint
Financial Sponsor: NASA Marshall Space Flight Center; Huntsville, AL United States
Organization Source: NASA Marshall Space Flight Center; Huntsville, AL United States
Description: 1p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: No Copyright
Availability Source: Other Sources
Availability Notes: Abstract Only
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