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Record Details

Record 29 of 1343
Use of Capillaries for Macromolecular Crystallization in a Cryogenic Dewar
Author and Affiliation:
Ciszak, Ewa(NASA Marshall Space Flight Center, Huntsville, AL United States)
Hammons, Aaron S.(Universities Space Research Association, Huntsville, AL United States)
Hong, Young Soo(Universities Space Research Association, Huntsville, AL United States)
Abstract: The enhanced gaseous nitrogen (EGN) dewar is a cryogenic dry shipper with a sealed cylinder inserted inside along with a temperature monitoring device, and is intended for macromolecular crystallization experiments on the International Space Station. Within the dewar, each crystallization experiment is contained as a solution within a plastic capillary tube. The standard procedure for loading samples in these tubes has involved rapid freezing of the precipitant and biomolecular solution, e.g., protein, directly in liquid nitrogen; this method, however, often resulted in uncontrolled formation of air voids, These air pockets, or bubbles, can lead to irreproducible crystallization results. A novel protocol has been developed to prevent formation of bubbles, and this has been tested in the laboratory as well as aboard the International Space Station during a 42-day long mission of July/August 2001. The gain or loss of mass from solutions within the plastic capillaries revealed that mass transport occurred among separated tubes, and that this mass transport was dependent upon the hygroscopic character of the solution contained in any given tube. The surface area of the plastic capillary tube also related to the observed mass transport. Furthermore, the decreased mass of solutions of-protein correlated to observed formation of protein crystals.
Publication Date: Jan 01, 2002
Document ID:
20030014040
(Acquired Feb 28, 2003)
Subject Category: SOLID-STATE PHYSICS
Document Type: Reprint
Publication Information: Crystal Growth and Design; Volume 2; No. 3; 235-238
Publisher Information: American Chemical Society, United States
Contract/Grant/Task Num: NAG8-1841; NSSTC-004
Financial Sponsor: NASA Marshall Space Flight Center; Huntsville, AL United States
National Space Science and Technology Center; Huntsville, AL United States
NASA Langley Research Center; Hampton, VA United States
Organization Source: NASA Marshall Space Flight Center; Huntsville, AL United States
Description: 6p; In English; Original contains color illustrations
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: CAPILLARY TUBES; CRYSTALLIZATION; CRYOGENIC EQUIPMENT; HYGROSCOPICITY; NITROGEN; BUBBLES; INTERNATIONAL SPACE STATION; MASS TRANSFER; SPACEBORNE EXPERIMENTS
Availability Source: Other Sources
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