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Physiological and Molecular Genetic Effects of Time-Varying Electromagnetic Fields on Human Neuronal CellsThe present investigation details the development of model systems for growing two- and three-dimensional human neural progenitor cells within a culture medium facilitated by a time-varying electromagnetic field (TVEMF). The cells and culture medium are contained within a two- or three-dimensional culture vessel, and the electromagnetic field is emitted from an electrode or coil. These studies further provide methods to promote neural tissue regeneration by means of culturing the neural cells in either configuration. Grown in two dimensions, neuronal cells extended longitudinally, forming tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time-varying electrical current was conducted. In the three-dimensional aspect, exposure to TVEMF resulted in the development of three-dimensional aggregates, which emulated organized neural tissues. In both experimental configurations, the proliferation rate of the TVEMF cells was 2.5 to 4.0 times the rate of the non-waveform cells. Each of the experimental embodiments resulted in similar molecular genetic changes regarding the growth potential of the tissues as measured by gene chip analyses, which measured more than 10,000 human genes simultaneously.
Document ID
20030075722
Acquisition Source
Johnson Space Center
Document Type
Technical Publication (TP)
Authors
Goodwin, Thomas J.
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
September 7, 2013
Publication Date
September 1, 2003
Subject Category
Life Sciences (General)
Report/Patent Number
NASA/TP-2003-212054
S-906
NAS 1.60:212054
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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