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Osteoblast Differentiation Decreases Hypergravity-Stimulated Release of PGE(sub 2)We determined if progressive differentiation of osteoblasts influences their sensitivity to gravitational loading. Osteoblasts were cultured for 4 days (confluent monolayer), 6 days (prenodules), 9 days (nodules) and 19 days (mineralized nodules), then centrifuged at 10 times gravity (g) or 50-g for 3 hours using the NASA Ames 1-ft. Diameter Centrifuge. Stationary controls were placed in an adjacent incubator. Following centrifugation, conditioned media were collected and analyzed for PGE, by ELISA. Microtubules were fluorescently labeled and analyzed by confocal microscopy to determine microtubule network morphology and height. Centrifugation at 10-g reduced microtubule network height by 15% on d4 and 10% on d6, with variable changes in more mature cultures. No major changes in microtubule morphology were observed. PGE(sub 2) release by d4 cultures increased in a dose-dependent fashion (3-fold at 10-g and 6-fold at 50-g relative to controls). D6 cultures produced a 5-fold increase for both 10-g and 50-g. PGE(sub 2) increased only 1.5-fold by d9, and by d19, PGE(sub 2) was not delectable in either the control or hypergravity-stimulated cells. Thus, as osteoblasts differentiate in culture, responsiveness of the microtubule cytoskeleton and the PGE(sub 2) pathway to hypergravity declines.
Document ID
20040053268
Document Type
Conference Paper
Authors
Searby, Nancy D. (NASA Ames Research Center Moffett Field, CA, United States)
Steele, Charles R. (Stanford Univ. Stanford, CA, United States)
Globus, Ruth K. (NASA Ames Research Center Moffett Field, CA, United States)
Date Acquired
August 21, 2013
Publication Date
November 4, 2002
Subject Category
Life Sciences (General)
Meeting Information
American Society of Gravitational and Space Biology Meeting
Funding Number(s)
CONTRACT_GRANT: NAGW-4625
CONTRACT_GRANT: 99-HEDS-02
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.