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X-ray Microscopic Characterization of Protein CrystalsThe microscopic mapping of the variation in degree of perfection and in type of defects in entire protein crystals by x-rays may well be a prerequisite for better understanding causes of lattice imperfections, the growth history, and properties of protein crystals. However, x-ray microscopic characterization of bulk protein crystals, in the as-grown state, is frequently more challenging than that of small molecular crystals due to the experimental difficulties arising largely from the unique features possessed by protein crystals. In this presentation, we will illustrate ssme recent activities in employing coherence-based phase contrast x-ray imaging and high-angular-resolution diffraction techniques for mapping microdefects and the degree of perfection of protein crystals, and demonstrate a correlation between crystal perfection, diffraction phenomena., and crystallization conditions. The observed features and phenomena will be discussed in context to gain insight into the nature of defects, nucleation and growth, and the properties of protein crystals.
Document ID
20040082221
Acquisition Source
Marshall Space Flight Center
Document Type
Conference Paper
Authors
Hu, Z. W.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Holmes, A.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Thomas, B.R.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Chernov, a. A.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Chu, Y. S.
(Argonne National Lab. IL, United States)
Lai, B.
(Argonne National Lab. IL, United States)
Date Acquired
August 21, 2013
Publication Date
January 1, 2004
Subject Category
Instrumentation And Photography
Meeting Information
Meeting: 10th International Conference on the Crystallization of Biological Maromolecules (ICCBM)
Location: Beijing
Country: China
Start Date: June 5, 2004
End Date: June 8, 2004
Funding Number(s)
CONTRACT_GRANT: NAS8-02096
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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