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Purification and Crystallization of Murine Myostatin: A Negative Regulator of Muscle MassMyostatin (MSTN) has been crystallized and its preliminary X-ray diffraction data were collected. MSTN is a negative regulator of muscle growt/differentiation and suppressor of fat accumulation. It is a member of TGF-b family of proteins. Like other members of this family, the regulation of MSTN is critically tied to its process of maturation. This process involves the formation of a homodimer followed by two proteolytic steps. The first proteolytic cleavage produces a species where the n-terminal portion of the dimer is covalently separated from, but remains non-covalently bound to, the c-terminal, functional, portion of the protein. The protein is activated upon removal of the n-terminal "pro-segment" by a second n-terminal proteolytic cut by BMP-1 in vivo, or by acid treatment in vitro. Understanding the structural nature and physical interactions involved in these regulatory processes is the objective of our studies. Murine MSTN was purified from culture media of genetically engineered Chinese Hamster Ovary cells by multicolumn purification process and crystallized using the vapor diffusion method.
Document ID
20040082222
Document Type
Preprint (Draft being sent to journal)
Authors
Hong, Young S. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Adamek, Daniel (NASA Marshall Space Flight Center Huntsville, AL, United States)
Bridge, Kristi (NASA Marshall Space Flight Center Huntsville, AL, United States)
Malone, Christine C. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Young, Ronald B. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Miller, Teresa (NASA Marshall Space Flight Center Huntsville, AL, United States)
Karr, Laurel (NASA Marshall Space Flight Center Huntsville, AL, United States)
Date Acquired
August 21, 2013
Publication Date
January 1, 2004
Subject Category
Life Sciences (General)
Meeting Information
American Society for Biochemistry and Molecular Biology(Boston, MA)
Funding Number(s)
CONTRACT_GRANT: NAS8-02096
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.