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Alteration of gene expression profiles in skeletal muscle of rats exposed to microgravity during a spaceflightTo clarify the mechanism of skeletal muscle wasting during spaceflights, we investigated whether intramuscular gene expression profiles are affected, by using DNA microarray methods. Male rats sent on the 17-day NASA STS-90 Neurolab spaceflight were sacrificed 24 hours after return to earth (MG group). Ground control rats were maintained for 17 days in flight-simulated cages (CS group). Spaceflight induced a 19% and 23% loss of tibialis anterior and gastrocnemius muscle mass, respectively, as compared to ground controls. Muscle RNA was analyzed by the Clontech Atlas DNA expression array in four rats, with two MG/ CS pairs for the tibialis anterior, and one pair for the gastrocnemius. Alterations in gene expression were verified for selected genes by reverse-transcription PCR. In both muscles of MG rats, mRNAs for 12 genes were up-regulated by over 2-fold, and 38 were down-regulated compared to controls. There was inhibition of genes for cell proliferation and growth factor cascades, including cell cycle genes and signal transduction proteins, such as p21 Cip1, retinoblastoma (Rb), cyclins G1/S, -E and -D3, MAP kinase 3, MAD3, and ras related protein RAB2. These data indicate that following exposure to microgravity, there is downregulation of genes involved in regulation of muscle satellite cell replication.
Document ID
20040087468
Document Type
Reprint (Version printed in journal)
Authors
Taylor, Wayne E. (Charles R Drew University of Medicine and Science Los Angeles, CA, United States)
Bhasin, Shalender
Lalani, Rukhsana
Datta, Anuj
Gonzalez-Cadavid, Nestor F.
Date Acquired
August 21, 2013
Publication Date
December 1, 2002
Publication Information
Publication: Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology
Volume: 9
Issue: 2
ISSN: 1077-9248
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 1RO1AG14369-01
CONTRACT_GRANT: 2R01DK49296-06
CONTRACT_GRANT: RCMI P20RR11145
CONTRACT_GRANT: 1RO1DK59627-01
CONTRACT_GRANT: RCMI G12RR03026
CONTRACT_GRANT: FDA ODP-00001397
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program Fundamental Space Biology
Non-NASA Center
STS-90 Shuttle Project
NASA Discipline Cell Biology
Flight Experiment
short duration
manned