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Effects of diet and exposure to hindlimb suspension on estrous cycling in Sprague-Dawley ratsVarious factors can disrupt the female reproductive cycle resulting in subfertility. The primary objective of this study was to determine whether physiological changes associated with exposure to hypogravity disrupt reproductive cycles. The hindlimb suspension (HLS) model was used to simulate the major physiological effects of hypogravity in female Sprague-Dawley rats. Also, to determine whether diet may influence reproductive results, rats were fed purified American Institute of Nutrition (AIN)-93G or chow diet. Rats (n = 9-11/group) subjected to HLS had lengthened estrous cycles due to prolonged diestrus, indicating hypoestrogenism. Interestingly, HLS rats fed AIN-93G but not chow diet had significantly reduced time spent in estrus and decreased plasma estradiol. Attenuation of hypoestrogenism in the chow-fed rats suggested that diet provided an exogenous source of estrogen. The mechanism involved in the disruption of estrous cycling remains to be determined. HLS increased urinary corticosterone (CORT) levels during the initial 4 days of HLS, suggesting that physiological responses to acute stress may be a potential mechanism in the disruption of estrous cycles. Higher basal urinary CORT was observed in rats fed chow vs. AIN-93G diet. HLS resulted in increased urinary CORT. However, two-way ANOVA indicated a significant HLS effect (P < 0.001) but no effect of HLS x diet effect on urinary CORT levels, suggesting that estrogenic activity associated with the chow diet did not enhance the stress response. The results of this study indicate that HLS, diet, and the combination of HLS and diet influence estrous cycling. This has important implications for future reproductive success in the hypogravity environment of space.
Document ID
20040087476
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Tou, Janet C L.
(NASA Ames Research Center Moffett Field CA United States)
Grindeland, Richard E.
Wade, Charles E.
Date Acquired
August 21, 2013
Publication Date
March 1, 2004
Publication Information
Publication: American journal of physiology. Endocrinology and metabolism
Volume: 286
Issue: 3
ISSN: 0193-1849
Subject Category
Aerospace Medicine
Distribution Limits
Public
Copyright
Other

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