NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Chemically defined medium and Caenorhabditis elegansBACKGROUND: C. elegans has been established as a powerful genetic system. Use of a chemically defined medium (C. elegans Maintenance Medium (CeMM)) now allows standardization and systematic manipulation of the nutrients that animals receive. Liquid cultivation allows automated culturing and experimentation and should be of use in large-scale growth and screening of animals. RESULTS: We find that CeMM is versatile and culturing is simple. CeMM can be used in a solid or liquid state, it can be stored unused for at least a year, unattended actively growing cultures may be maintained longer than with standard techniques, and standard C. elegans protocols work well with animals grown in defined medium. We also find that there are caveats to using defined medium. Animals in defined medium grow more slowly than on standard medium, appear to display adaptation to the defined medium, and display altered growth rates as they change the composition of the defined medium. CONCLUSIONS: As was suggested with the introduction of C. elegans as a potential genetic system, use of defined medium with C. elegans should prove a powerful tool.
Document ID
20040087483
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Szewczyk, Nathaniel J.
(NASA Ames Research Center Moffett Field CA United States)
Kozak, Elena
Conley, Catharine A.
Date Acquired
August 21, 2013
Publication Date
October 27, 2003
Publication Information
Publication: BMC biotechnology [electronic resource]
Volume: 3
Issue: 1
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other

Available Downloads

There are no available downloads for this record.
No Preview Available