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Record Details

Record 38 of 2432
The potato virus X TGBp2 protein association with the endoplasmic reticulum plays a role in but is not sufficient for viral cell-to-cell movement
External Online Source: doi:10.1016/S0042-6822(03)00180-6
Author and Affiliation:
Mitra, Ruchira(Oklahoma State University, Department of Entomology and Plant Pathology, NRC 127, Stillwater, OK 74078, United States)
Krishnamurthy, Konduru
Blancaflor, Elison
Payton, Mark
Nelson, Richard S.
Verchot-Lubicz, Jeanmarie
Abstract: Potato virus X (PVX) TGBp1, TGBp2, TGBp3, and coat protein are required for virus cell-to-cell movement. Plasmids expressing GFP fused to TGBp2 were bombarded to leaf epidermal cells and GFP:TGBp2 moved cell to cell in Nicotiana benthamiana leaves but not in Nicotiana tabacum leaves. GFP:TGBp2 movement was observed in TGBp1-transgenic N. tabacum, indicating that TGBp2 requires TGBp1 to promote its movement in N. tabacum. In this study, GFP:TGBp2 was detected in a polygonal pattern that resembles the endoplasmic reticulum (ER) network. Amino acid sequence analysis revealed TGBp2 has two putative transmembrane domains. Two mutations separately introduced into the coding sequences encompassing the putative transmembrane domains within the GFP:TGBp2 plasmids and PVX genome, disrupted membrane binding of GFP:TGBp2, inhibited GFP:TGBp2 movement in N. benthamiana and TGBp1-expressing N. tabacum, and inhibited PVX movement. A third mutation, lying outside the transmembrane domains, had no effect on GFP:TGBp2 ER association or movement in N. benthamiana but inhibited GFP:TGBp2 movement in TGBp1-expressing N. tabacum and PVX movement in either Nicotiana species. Thus, ER association of TGBp2 may be required but not be sufficient for virus movement. TGBp2 likely provides an activity for PVX movement beyond ER association.
Publication Date: Jul 20, 2003
Document ID:
20040087575
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Virology (ISSN 0042-6822); Volume 312; 1; 35-48
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ENDOPLASMIC RETICULUM; MOTION; POTATOES; PROTEINS; VIRUSES; AMINO ACIDS; CYTOLOGY; EXOBIOLOGY; GENE EXPRESSION REGULATION; GENETICALLY MODIFIED PLANTS; GENETICS; LEAVES; LUMINESCENT PROTEINS; METABOLISM; MUTATIONS; PLANTS (BOTANY); TOBACCO
Other Descriptors: ENDOPLASMIC RETICULUM/METABOLISM; MOVEMENT; POTEXVIRUS/GENETICS/PHYSIOLOGY; VIRAL PROTEINS/CHEMISTRY/GENETICS/METABOLISM; AMINO ACID SEQUENCE; GENE EXPRESSION REGULATION, VIRAL; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PLANT LEAVES/CYTOLOGY/METABOLISM/VIROLOGY; PLANTS, GENETICALLY MODIFIED; POTATOES/VIROLOGY; PROTEIN TRANSPORT; SEQUENCE ALIGNMENT; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; TOBACCO/CYTOLOGY/METABOLISM/VIROLOGY; NASA DISCIPLINE PLANT BIOLOGY; NASA PROGRAM FUNDAMENTAL SPACE BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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