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Tetraspanin CD151 regulates alpha6beta1 integrin adhesion strengtheningThe tetraspanin CD151 molecule associates specifically with laminin-binding integrins, including alpha6beta1. To probe strength of alpha6beta1-dependent adhesion to laminin-1, defined forces (0-1.5 nN) were applied to magnetic laminin-coated microbeads bound to NIH 3T3 cells. For NIH 3T3 cells bearing wild-type CD151, adhesion strengthening was observed, as bead detachment became more difficult over time. In contrast, mutant CD151 (with the C-terminal region replaced) showed impaired adhesion strengthening. Static cell adhesion to laminin-1, and detachment of beads coated with fibronectin or anti-alpha6 antibody were all unaffected by CD151 mutation. Hence, CD151 plays a key role in selectively strengthening alpha6beta1 integrin-mediated adhesion to laminin-1.
Document ID
20040087635
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Lammerding, Jan (Massachusetts Institute of Technology Cambridge, MA 02139, United States)
Kazarov, Alexander R.
Huang, Hayden
Lee, Richard T.
Hemler, Martin E.
Date Acquired
August 21, 2013
Publication Date
June 24, 2003
Publication Information
Publication: Proceedings of the National Academy of Sciences of the United States of America
Volume: 100
Issue: 13
ISSN: 0027-8424
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: PO1-HL64858
CONTRACT_GRANT: R01-CA42368
CONTRACT_GRANT: R01-CA86712
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Cell Biology
Non-NASA Center