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Radiation-induced gene expression in the nematode Caenorhabditis elegansWe used the nematode C. elegans to characterize the genotoxic and cytotoxic effects of ionizing radiation in a simple animal model emphasizing the unique effects of charged particle radiation. Here we demonstrate by RT-PCR differential display and whole genome microarray hybridization experiments that gamma rays, accelerated protons and iron ions at the same physical dose lead to unique transcription profiles. 599 of 17871 genes analyzed (3.4%) showed differential expression 3 hrs after exposure to 3 Gy of radiation. 193 were up-regulated, 406 were down-regulated and 90% were affected only by a single species of radiation. A novel statistical clustering technique identified the regulatory relationships between the radiation-modulated genes and showed that genes affected by each radiation species were associated with unique regulatory clusters. This suggests that independent homeostatic mechanisms are activated in response to radiation exposure as a function of track structure or ionization density.
Document ID
20040087647
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Nelson, Gregory A.
(Loma Linda University Radiobiology Program 11175 Campus St., Chan Shun Pavilion A1010 Loma Linda, California 92354, United States)
Jones, Tamako A.
Chesnut, Aaron
Smith, Anna L.
Date Acquired
August 21, 2013
Publication Date
December 1, 2002
Publication Information
Publication: Journal of radiation research
Volume: 43 Suppl
ISSN: 0449-3060
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

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