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Chronic shear induces caveolae formation and alters ERK and Akt responses in endothelial cellsCaveolae are plasmalemmal domains enriched with cholesterol, caveolins, and signaling molecules. Endothelial cells in vivo are continuously exposed to shear conditions, and their caveolae density and location may be different from that of static cultured cells. Here, we show that chronic shear exposure regulates formation and localization of caveolae and caveolin-1 in bovine aortic endothelial cells (BAEC). Chronic exposure (1 or 3 days) of BAEC to laminar shear increased the total number of caveolae by 45-48% above static control. This increase was due to a rise in the luminal caveolae density without changing abluminal caveolae numbers or increasing caveolin-1 mRNA and protein levels. Whereas some caveolin-1 was found in the plasma membrane in static-cultured cells, it was predominantly localized in the Golgi. In contrast, chronic shear-exposed cells showed intense caveolin-1 staining in the luminal plasma membrane with minimum Golgi association. The preferential luminal localization of caveolae may play an important role in endothelial mechanosensing. Indeed, we found that chronic shear exposure (preconditioning) altered activation patterns of two well-known shear-sensitive signaling molecules (ERK and Akt) in response to a step increase in shear stress. ERK activation was blunted in shear preconditioned cells, whereas the Akt response was accelerated. These results suggest that chronic shear stimulates caveolae formation by translocating caveolin-1 from the Golgi to the luminal plasma membrane and alters cell signaling responses.
Document ID
20040087680
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Boyd, Nolan L.
(Wallace H. Coulter Dept. of Biomedical Engineering at Georgia Tech and Emory University Atlanta, GA 30322, United States)
Park, Heonyong
Yi, Hong
Boo, Yong Chool
Sorescu, George P.
Sykes, Michelle
Jo, Hanjoong
Date Acquired
August 21, 2013
Publication Date
September 1, 2003
Publication Information
Publication: American journal of physiology. Heart and circulatory physiology
Volume: 285
Issue: 3
ISSN: 0363-6135
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: HL 67413
CONTRACT_GRANT: HL 71014
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Cell Biology
Non-NASA Center

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