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Record Details

Record 43 of 963
Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays
External Online Source: doi:10.1128/AEM.69.4.2377-2382.2003
Author and Affiliation:
El Fantroussi, Said(University of Washington, Department of Civil and Environmental Engineering, Seattle 98195, United States)
Urakawa, Hidetoshi
Bernhard, Anne E.
Kelly, John J.
Noble, Peter A.
Smidt, H.
Yershov, G. M.
Stahl, David A.
Abstract: Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.
Publication Date: Apr 01, 2003
Document ID:
20040087760
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Applied and environmental microbiology (ISSN 0099-2240); Volume 69; 4; 2377-82
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: BACTERIA; FRESH WATER; MICROORGANISMS; NUCLEIC ACIDS; OLIGONUCLEOTIDES; POPULATIONS; RIBONUCLEIC ACIDS; SEDIMENTS; THERMAL DISSOCIATION; SENSITIVITY ANALYSIS; TEMPERATURE
Other Descriptors: BACTERIA/GENETICS; FRESH WATER/MICROBIOLOGY; GEOLOGIC SEDIMENTS/MICROBIOLOGY; NUCLEIC ACID HYBRIDIZATION/METHODS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RNA, RIBOSOMAL/GENETICS; OLIGONUCLEOTIDE PROBES; SENSITIVITY AND SPECIFICITY; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; TEMPERATURE; EVALUATION STUDIES; NASA DISCIPLINE LIFE SCIENCES TECHNOLOGIES; NON-NASA CENTER
Availability Source: Other Sources
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