NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistantOne of the spore-formers isolated from a spacecraft-assembly facility, belonging to the genus Bacillus, is described on the basis of phenotypic characterization, 16S rDNA sequence analysis and DNA-DNA hybridization studies. It is a Gram-positive, facultatively anaerobic, rod-shaped eubacterium that produces endospores. The spores of this novel bacterial species exhibited resistance to UV, gamma-radiation, H2O2 and desiccation. The 18S rDNA sequence analysis revealed a clear affiliation between this strain and members of the low G+C Firmicutes. High 16S rDNA sequence similarity values were found with members of the genus Bacillus and this was supported by fatty acid profiles. The 16S rDNA sequence similarity between strain FO-92T and Bacillus benzoevorans DSM 5391T was very high. However, molecular characterizations employing small-subunit 16S rDNA sequences were at the limits of resolution for the differentiation of species in this genus, but DNA-DNA hybridization data support the proposal of FO-92T as Bacillus nealsonii sp. nov. (type strain is FO-92T =ATCC BAAM-519T =DSM 15077T).
Document ID
20040087773
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Venkateswaran, Kasthuri
(Jet Propulsion Lab., California Inst. of Tech. Pasadena CA United States)
Kempf, Michael
Chen, Fei
Satomi, Masataka
Nicholson, Wayne
Kern, Roger
Date Acquired
August 21, 2013
Publication Date
January 1, 2003
Publication Information
Publication: International journal of systematic and evolutionary microbiology
Volume: 53
Issue: Pt 1
ISSN: 1466-5026
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-programmatic

Available Downloads

There are no available downloads for this record.
No Preview Available