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Are chromosomal instabilities induced by exposure of cultured normal human cells to low- or high-LET radiation?Radiation-induced genomic instability has been proposed as a very early, if not an initiating, step in radiation carcinogenesis. Numerous studies have established the occurrence of radiation-induced chromosomal instability in various cells of both human and rodent origin. In many of these studies, however, the cells were not "normal" initially, and in many cases they involved tumor-derived cell lines. The phenomenon clearly would be of even greater interest if it were shown to occur generally in cells that are normal at the outset, rather than cells that may have been "selected" because of a pre-existing susceptibility to induced instability. As a test of the generality of the phenomenon, we studied low-passage normal diploid human fibroblasts (AG1521A) to determine whether they are susceptible to the induction of chromosomal instability in the progeny of surviving cells after exposure in G(0) to low- and high-LET radiation. Cytogenetic assays for instability were performed on both mixed populations of cells and clones of cells surviving exposure. We found no evidence for the induction of such instability as a result of radiation exposure, though we observed a senescence-related chromosomal instability in the progeny of both irradiated and unirradiated cell populations. Copyright 2003 by Radiation Research Society.
Document ID
20040087824
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Dugan, Lawrence C.
(Colorado State University Fort Collins, Colorado 80523, United States)
Bedford, Joel S.
Date Acquired
August 21, 2013
Publication Date
March 1, 2003
Publication Information
Publication: Radiation research
Volume: 159
Issue: 3
ISSN: 0033-7587
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: T32-CA09236
CONTRACT_GRANT: R01-CA73926
CONTRACT_GRANT: R01-CA80651
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

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