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Record 32 of 536
Influence of coronary artery diameter on eNOS protein content
Author and Affiliation:
Laughlin, M. H.(Department of Veterinary Biomedical Sciences and Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri 65211, United States)
Turk, J. R.
Schrage, W. G.
Woodman, C. R.
Price, E. M.
Abstract: The purpose of this study was to test the hypothesis that the content of endothelial nitric oxide synthase (eNOS) protein (eNOS protein/g total artery protein) increases with decreasing artery diameter in the coronary arterial tree. Content of eNOS protein was determined in porcine coronary arteries with immunoblot analysis. Arteries were isolated in six size categories from each heart: large arteries [301- to 2,500-microm internal diameter (ID)], small arteries (201- to 300-microm ID), resistance arteries (151- to 200-microm ID), large arterioles (101- to 150-microm ID), intermediate arterioles (51- to 100-microm ID), and small arterioles(<50-microm ID). To obtain sufficient protein for analysis from small- and intermediate-sized arterioles, five to seven arterioles 1-2 mm in length were pooled into one sample for each animal. Results establish that the number of smooth muscle cells per endothelial cell decreases from a number of 10 to 15 in large coronary arteries to 1 in the smallest arterioles. Immunohistochemistry revealed that eNOS is located only in endothelial cells in all sizes of coronary artery and in coronary capillaries. Contrary to our hypothesis, eNOS protein content did not increase with decreasing size of coronary artery. Indeed, the smallest coronary arterioles had less eNOS protein per gram of total protein than the large coronary arteries. These results indicate that eNOS protein content is greater in the endothelial cells of conduit arteries, resistance arteries, and large arterioles than in small coronary arterioles.
Publication Date: Apr 01, 2003
Document ID:
20040087831
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: American journal of physiology. Heart and circulatory physiology (ISSN 0363-6135); Volume 284; 4; H1307-12
Publisher Information: United States
Contract/Grant/Task Num: HL-09739; HL-52490
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ARTERIES; ENZYMES; HEART; PROTEINS; ARTERIOLES; CAPILLARIES (ANATOMY); CELLS (BIOLOGY); ENDOTHELIUM; FEMALES; HISTOCHEMICAL ANALYSIS; IMMUNOLOGY; PHYSIOCHEMISTRY; SMOOTH MUSCLE; SWINE; VEINS
Other Descriptors: CORONARY VESSELS/ANATOMY & HISTOLOGY/ENZYMOLOGY; NITRIC-OXIDE SYNTHASE/ANALYSIS; ANIMALS; ARTERIOLES/ANATOMY & HISTOLOGY/ENZYMOLOGY; CAPILLARIES/ENZYMOLOGY; CELL COUNT; ENDOTHELIUM, VASCULAR/CYTOLOGY; FEMALE; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MUSCLE, SMOOTH, VASCULAR/CYTOLOGY; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; SWINE, MINIATURE; VEINS/ENZYMOLOGY; NASA DISCIPLINE CARDIOPULMONARY; NON-NASA CENTER
Availability Source: Other Sources
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