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Interactions between neutrophils and macrophages promote macrophage killing of rat muscle cells in vitroCurrent evidence indicates that the physiological functions of inflammatory cells are highly sensitive to their microenvironment, which is partially determined by the inflammatory cells and their potential targets. In the present investigation, interactions between neutrophils, macrophages and muscle cells that may influence muscle cell death are examined. Findings show that in the absence of macrophages, neutrophils kill muscle cells in vitro by superoxide-dependent mechanisms, and that low concentrations of nitric oxide (NO) protect against neutrophil-mediated killing. In the absence of neutrophils, macrophages kill muscle cells through a NO-dependent mechanism, and the presence of target muscle cells causes a three-fold increase in NO production by macrophages, with no change in the concentration of inducible nitric oxide synthase. Muscle cells that are co-cultured with both neutrophils and macrophages in proportions that are observed in injured muscle show cytotoxicity through a NO-dependent, superoxide-independent mechanism. Furthermore, the concentration of myeloid cells that is necessary for muscle killing is greatly reduced in assays that use mixed myeloid cell populations, rather than uniform populations of neutrophils or macrophages. These findings collectively show that the magnitude and mechanism of muscle cell killing by myeloid cells are modified by interactions between muscle cells and neutrophils, between muscle cells and macrophages and between macrophages and neutrophils.
Document ID
20040087870
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Nguyen, Hal X.
(University of California Los Angeles, CA 90095, United States)
Tidball, James G.
Date Acquired
August 21, 2013
Publication Date
February 15, 2003
Publication Information
Publication: The Journal of physiology
Volume: 547
Issue: Pt 1
ISSN: 0022-3751
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: AR47855-01
CONTRACT_GRANT: AR40343
CONTRACT_GRANT: AR
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Musculoskeletal

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