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Record 1 of 6818
Toll-like receptor 4-positive macrophages protect mice from Pasteurella pneumotropica-induced pneumonia
External Online Source: doi:10.1128/IAI.71.2.663-670.2003
Author and Affiliation:
Hart, Marcia L.(Kansas State University, Division of Biology, Manhattan 66506, United States)
Mosier, Derek A.
Chapes, Stephen K.
Abstract: This study investigates Toll-like receptor 4 (TLR4)-positive macrophages in early recognition and clearance of pulmonary bacteria. TLR4 is a trans-membrane receptor that is the primary recognition molecule for lipopolysaccharide of gram-negative bacteria. The TLR4(Lps-del) mouse strains C57BL10/ScN (B10) and STOCK Abb(tm1) TLR4(Lps-del) Slc11a1(s)(B10 x C2D) are susceptible to pulmonary infections and develop pneumonia when naturally or experimentally infected by the opportunistic bacterium Pasteurella pneumotropica. Since these mice have the TLR4(Lps-del) genotype, we hypothesized that reconstitution of mice with TLR4-positive macrophages would provide resistance to this bacterium. A cultured macrophage cell line (C2D macrophages) and bone marrow cells from C2D mice were adoptively transferred to B10 and B10 x C2D mice by intraperitoneal injection. C2D macrophages increased B10 and B10 x C2D mouse resistance to P. pneumotropica. In C2D-recipient mice there was earlier transcription of tumor necrosis factor alpha and chemokines JE and macrophage inflammatory protein 2 (MIP-2) in the lungs of B10 and B10 x C2D mice, and there was earlier transcription of KC and MIP-1alpha in B10 x C2D mice. In addition, the course of inflammation following experimental Pasteurella challenge was altered in C2D recipients. C2D macrophages also protected B10 x C2D mice, which lack CD4(+) T cells. These data indicate that macrophages are critical for pulmonary immunity and can provide host resistance to P. pneumotropica. This study indicates that TLR4-positive macrophages are important for early recognition and clearance of pulmonary bacterial infections.
Publication Date: Feb 01, 2003
Document ID:
20040087889
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Infection and immunity (ISSN 0019-9567); Volume 71; 2; 663-70
Publisher Information: United States
Contract/Grant/Task Num: AI050785; RR16475
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: BACTERIA; BACTERIAL DISEASES; IMMUNOLOGY; MACROPHAGES; MICE; PNEUMONIA; CULTURED CELLS; GENES; GENETICS; METABOLISM; MICROBIOLOGY
Other Descriptors: DROSOPHILA PROTEINS; MACROPHAGES/IMMUNOLOGY/METABOLISM/TRANSPLANTATION; MEMBRANE GLYCOPROTEINS/DEFICIENCY/GENETICS/IMMUNOLOGY/METABOLISM; PASTEURELLA/PATHOGENICITY; PNEUMONIA, BACTERIAL/IMMUNOLOGY/MICROBIOLOGY/PREVENTION & CONTROL; RECEPTORS, CELL SURFACE/DEFICIENCY/GENETICS/IMMUNOLOGY/METABOLISM; ADOPTIVE TRANSFER; ANIMALS; CELL LINE; CROSSES, GENETIC; GENES, MHC CLASS II; GENETIC PREDISPOSITION TO DISEASE; MICE; MICE, INBRED C57BL; PASTEURELLA INFECTIONS/IMMUNOLOGY/MICROBIOLOGY/PREVENTION & CONTROL; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE CELL BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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