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Characteristics of genomic instability in clones of TK6 human lymphoblasts surviving exposure to 56Fe ionsGenomic instability in the human lymphoblast cell line TK6 was studied in clones surviving 36 generations after exposure to accelerated 56Fe ions. Clones were assayed for 20 characteristics, including chromosome aberrations, plating efficiency, apoptosis, cell cycle distribution, response to a second irradiation, and mutant frequency at two loci. The primary effect of the 56Fe-ion exposure on the surviving clones was a significant increase in the frequency of unstable chromosome aberrations compared to the very low spontaneous frequency, along with an increase in the phenotypic complexity of the unstable clones. The radiation-induced increase in the frequency of unstable chromosome aberrations was much greater than that observed previously in clones of the related cell line, WTK1, which in comparison to the TK6 cell line expresses an increased radiation resistance, a mutant TP53 protein, and an increased frequency of spontaneous unstable chromosome aberrations. The characteristics of the unstable clones of the two cell lines also differed. Most of the TK6 clones surviving exposure to 56Fe ions showed unstable cytogenetic abnormalities, while the phenotype of the WTK1 clones was more diverse. The results underscore the importance of genotype in the characteristics of instability after radiation exposure.
Document ID
20040087962
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Evans, Helen H.
(Case Western Reserve University Cleveland, Ohio 44106-4942, United States)
Horng, Min-Fen
Ricanati, Marlene
Diaz-Insua, Mireya
Jordan, Robert
Schwartz, Jeffrey L.
Date Acquired
August 21, 2013
Publication Date
December 1, 2002
Publication Information
Publication: Radiation research
Volume: 158
Issue: 6
ISSN: 0033-7587
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA 73931
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Radiation Health
Non-NASA Center

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