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Specimen block counter-staining for localization of GUS expression in transgenic arabidopsis and tobaccoA simple counter-staining procedure has been developed for comparative beta-glucuronidase (GUS) expression and anatomical localization in transgenic herbaceous arabidopsis and tobacco. This protocol provides good anatomical visualization for monitoring chimeric gene expression at both the organ and tissue levels. It can be used with different histochemical stains and can be extended to the study of woody species. The specimens are paraffin-embedded, the block is trimmed to reveal internal structure, safranin-O staining solution is briefly applied to the surface of the block, then washed off and, after drying, a drop of immersion oil is placed on the stained surface for subsequent photographic work. This gives tissue counter-staining with good structural preservation without loss of GUS staining product; moreover, sample observation is rapid and efficient compared to existing procedures.
Document ID
20040087976
Acquisition Source
Ames Research Center
Document Type
Reprint (Version printed in journal)
Authors
Kim, M. K.
(Institute of Biological Chemistry, Washington State University Pullman, WA 99164-6340, United States)
Choi, J-W
Jeon, J-H
Franceschi, V. R.
Davin, L. B.
Lewis, N. G.
Date Acquired
August 21, 2013
Publication Date
July 1, 2002
Publication Information
Publication: Plant cell reports
Volume: 21
Issue: 1
ISSN: 0721-7714
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAG2-1198
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Plant Biology
Non-NASA Center

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