NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Tissue engineering skeletal muscle for orthopaedic applicationsWith current technology, tissue-engineered skeletal muscle analogues (bioartificial muscles) generate too little active force to be clinically useful in orthopaedic applications. They have been engineered genetically with numerous transgenes (growth hormone, insulinlike growth factor-1, erythropoietin, vascular endothelial growth factor), and have been shown to deliver these therapeutic proteins either locally or systemically for months in vivo. Bone morphogenetic proteins belonging to the transforming growth factor-beta superfamily are osteoinductive molecules that drive the differentiation pathway of mesenchymal cells toward the chondroblastic or osteoblastic lineage, and stimulate bone formation in vivo. To determine whether skeletal muscle cells endogenously expressing bone morphogenetic proteins might serve as a vehicle for systemic bone morphogenetic protein delivery in vivo, proliferating skeletal myoblasts (C2C12) were transduced with a replication defective retrovirus containing the gene for recombinant human bone morphogenetic protein-6 (C2BMP-6). The C2BMP-6 cells constitutively expressed recombinant human bone morphogenetic protein-6 and synthesized bioactive recombinant human bone morphogenetic protein-6, based on increased alkaline phosphatase activity in coincubated mesenchymal cells. C2BMP-6 cells did not secrete soluble, bioactive recombinant human bone morphogenetic protein-6, but retained the bioactivity in the cell layer. Therefore, genetically-engineered skeletal muscle cells might serve as a platform for long-term delivery of osteoinductive bone morphogenetic proteins locally.
Document ID
20040088021
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Payumo, Francis C.
(Brown University School of Medicine/The Miriam Hospital 164 Summit Avenue, Providence, RI 02906, United States)
Kim, Hyun D.
Sherling, Michael A.
Smith, Lee P.
Powell, Courtney
Wang, Xiao
Keeping, Hugh S.
Valentini, Robert F.
Vandenburgh, Herman H.
Date Acquired
August 21, 2013
Publication Date
October 1, 2002
Publication Information
Publication: Clinical orthopaedics and related research
Issue: 403 Suppl
ISSN: 0009-921X
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: AG 15415
Distribution Limits
Public
Copyright
Other
Keywords
Review
Non-NASA Center
NASA Discipline Musculoskeletal
Review, Tutorial

Available Downloads

There are no available downloads for this record.
No Preview Available