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Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension cultureThis study utilizes Saccharomyces cerevisiae to study genetic responses to suspension culture. The suspension culture system used in this study is the high-aspect-ratio vessel, one type of the rotating wall vessel, that provides a high rate of gas exchange necessary for rapidly dividing cells. Cells were grown in the high-aspect-ratio vessel, and DNA microarray and metabolic analyses were used to determine the resulting changes in yeast gene expression. A significant number of genes were found to be up- or downregulated by at least twofold as a result of rotational growth. By using Gibbs promoter alignment, clusters of genes were examined for promoter elements mediating these genetic changes. Candidate binding motifs similar to the Rap1p binding site and the stress-responsive element were identified in the promoter regions of differentially regulated genes. This study shows that, as in higher order organisms, S. cerevisiae changes gene expression in response to rotational culture and also provides clues for investigations into the signaling pathways involved in gravitational response.
Document ID
Document Type
Reprint (Version printed in journal)
Johanson, Kelly (Tulane University Health Sciences Center New Orleans, Louisiana 70112, United States)
Allen, Patricia L.
Lewis, Fawn
Cubano, Luis A.
Hyman, Linda E.
Hammond, Timothy G.
Date Acquired
August 21, 2013
Publication Date
December 1, 2002
Publication Information
Publication: Journal of applied physiology (Bethesda, Md. : 1985)
Volume: 93
Issue: 6
ISSN: 8750-7587
Subject Category
Life Sciences (General)
Distribution Limits
Non-NASA Center
NASA Discipline Cell Biology