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Biochemical basis for the biological clockNADH oxidases at the external surface of plant and animal cells (ECTO-NOX proteins) exhibit stable and recurring patterns of oscillations with potentially clock-related, entrainable, and temperature-compensated period lengths of 24 min. To determine if ECTO-NOX proteins might represent the ultradian time keepers (pacemakers) of the biological clock, COS cells were transfected with cDNAs encoding tNOX proteins having a period length of 22 min or with C575A or C558A cysteine to alanine replacements having period lengths of 36 or 42 min. Here we demonstrate that such transfectants exhibited 22, 36, or 40 to 42 h circadian patterns in the activity of glyceraldehyde-3-phosphate dehydrogenase, a common clock-regulated protein, in addition to the endogenous 24 h circadian period length. The fact that the expression of a single oscillatory ECTO-NOX protein determines the period length of a circadian biochemical marker (60 X the ECTO-NOX period length) provides compelling evidence that ECTO-NOX proteins are the biochemical ultradian drivers of the cellular biological clock.
Document ID
20040088090
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Morre, D. James
(Purdue University West Lafayette, IN 47907, United States)
Chueh, Pin-Ju
Pletcher, Jake
Tang, Xiaoyu
Wu, Lian-Ying
Morre, Dorothy M.
Date Acquired
August 21, 2013
Publication Date
October 8, 2002
Publication Information
Publication: Biochemistry
Volume: 41
Issue: 40
ISSN: 0006-2960
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: P50 AT 00477
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Cell Biology
Non-NASA Center

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