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Microgravity modifies protein kinase C isoform translocation in the human monocytic cell line U937 and human peripheral blood T-cellsIndividual protein kinase C (PKC) isoforms fulfill distinct roles in the regulation of the commitment to differentiation, cell cycle arrest, and apoptosis in both monocytes and T-cells. The human monocyte like cell line U937 and T-cells were exposed to microgravity, during spaceflight and the translocation (a critical step in PKC signaling) of individual isoforms to cell particulate fraction examined. PKC activating phorbol esters induced a rapid translocation of several PKC isoforms to the particulate fraction of U937 monocytes under terrestrial gravity (1 g) conditions in the laboratory. In microgravity, the translocation of PKC beta II, delta, and epsilon in response to phorbol esters was reduced in microgravity compared to 1 g, but was enhanced in weak hypergravity (1.4 g). All isoforms showed a net increase in particulate PKC following phorbol ester stimulation, except PKC delta which showed a net decrease in microgravity. In T-cells, phorbol ester induced translocation of PKC delta was reduced in microgravity, compared to 1 g, while PKC beta II translocation was not significantly different at the two g-levels. These data show that microgravity differentially alters the translocation of individual PKC isoforms in monocytes and T-cells, thus providing a partial explanation for the modifications previously observed in the activation of these cell types under microgravity.
Document ID
20040088146
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Hatton, Jason P.
(Veterans Affairs Medical Center Mail Code 151F, San Francisco, California 94121, United States)
Gaubert, Francois
Cazenave, Jean-Pierre
Schmitt, Didier
Hashemi, B. B.
Hughes-Fulford, M.
Date Acquired
August 21, 2013
Publication Date
January 1, 2002
Publication Information
Publication: Journal of cellular biochemistry
Volume: 87
Issue: 1
ISSN: 0730-2312
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
STS-81 Shuttle Project
Flight Experiment
NASA Center JSC
NASA Discipline Cell Biology
manned
short duration
NASA Program Fundamental Space Biology
Non-NASA Center

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