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Record Details

Record 79 of 10267
Microgravity modifies protein kinase C isoform translocation in the human monocytic cell line U937 and human peripheral blood T-cells
External Online Source: doi:10.1002/jcb.10273
Author and Affiliation:
Hatton, Jason P.(Veterans Affairs Medical Center, Laboratory of Cell Growth, Mail Code 151F, San Francisco, California 94121, United States)
Gaubert, Francois
Cazenave, Jean-Pierre
Schmitt, Didier
Hashemi, B. B. [Principal Investigator]
Hughes-Fulford, M. [Principal Investigator]
Abstract: Individual protein kinase C (PKC) isoforms fulfill distinct roles in the regulation of the commitment to differentiation, cell cycle arrest, and apoptosis in both monocytes and T-cells. The human monocyte like cell line U937 and T-cells were exposed to microgravity, during spaceflight and the translocation (a critical step in PKC signaling) of individual isoforms to cell particulate fraction examined. PKC activating phorbol esters induced a rapid translocation of several PKC isoforms to the particulate fraction of U937 monocytes under terrestrial gravity (1 g) conditions in the laboratory. In microgravity, the translocation of PKC beta II, delta, and epsilon in response to phorbol esters was reduced in microgravity compared to 1 g, but was enhanced in weak hypergravity (1.4 g). All isoforms showed a net increase in particulate PKC following phorbol ester stimulation, except PKC delta which showed a net decrease in microgravity. In T-cells, phorbol ester induced translocation of PKC delta was reduced in microgravity, compared to 1 g, while PKC beta II translocation was not significantly different at the two g-levels. These data show that microgravity differentially alters the translocation of individual PKC isoforms in monocytes and T-cells, thus providing a partial explanation for the modifications previously observed in the activation of these cell types under microgravity.
Publication Date: Jan 01, 2002
Document ID:
20040088146
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Journal of cellular biochemistry (ISSN 0730-2312); Volume 87; 1; 39-50
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ENZYMES; LYMPHOCYTES; MICROGRAVITY; MONOCYTES; PROTEINS; CYTOMETRY; ELECTROPHORESIS; MODELS; SPACE SHUTTLE MISSIONS; TIME DEPENDENCE; WEIGHTLESSNESS
Other Descriptors: MONOCYTES/CYTOLOGY; PROTEIN KINASE C/CHEMISTRY/METABOLISM; SPACE FLIGHT; T-LYMPHOCYTES/CYTOLOGY; BLOTTING, WESTERN; FLOW CYTOMETRY; HUMAN; MODELS, BIOLOGICAL; PHORBOL ESTERS/PHARMACOLOGY; PROTEIN ISOFORMS; PROTEIN TRANSPORT; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; TIME FACTORS; U937 CELLS; WEIGHTLESSNESS; FLIGHT EXPERIMENT; STS-81 SHUTTLE PROJECT; MANNED; SHORT DURATION; NASA CENTER JSC; NASA DISCIPLINE CELL BIOLOGY; NASA PROGRAM FUNDAMENTAL SPACE BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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