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Record 3 of 31
Regulation of period 1 expression in cultured rat pineal
External Online Source: doi:10.1159/000058547
Author and Affiliation:
Fukuhara, Chiaki(Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA 30310-1495, United States)
Dirden, James C.
Tosini, Gianluca
Abstract: The aim of the present study was to investigate the in vitro expression of Period 1 (Per1), Period 2 (Per2) and arylalkylamine N-acetyltransferase (AA-NAT) genes in the rat pineal gland to understand the mechanism(s) regulating the expression of these genes in this organ. Pineals, when maintained in vitro for 5 days, did not show circadian rhythmicity in the expression of any of the three genes monitored. Norepinephrine (NE) induced AA-NAT and Per1, whereas its effect on Per2 was negligible. Contrary to what was observed in other systems, NE stimulation did not induce circadian expression of Per1. The effect of NE on Per1 level was dose- and receptor subtype-dependent, and both cAMP and cGMP induced Per1. Per1 was not induced by repeated NE - or forskolin - stimulation. Protein synthesis was not necessary for NE-induced Per1, but it was for reduction of Per1 following NE stimulation. Per1 transcription in pinealocytes was activated by BMAL1/CLOCK. Our results indicate that important differences are present in the regulation of these genes in the mammalian pineal. Copyright 2002 S. Karger AG, Basel.
Publication Date: Mar 01, 2002
Document ID:
20040088259
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Neuro-Signals (ISSN 1424-862X); Volume 11; 2; 103-14
Publisher Information: Switzerland
Contract/Grant/Task Num: NS 34194; NS 38483
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: GENE EXPRESSION REGULATION; PINEAL GLAND; RATS; ADRENERGICS; CIRCADIAN RHYTHMS; CYCLIC AMP; MALES; NOREPINEPHRINE; NUCLEOTIDES; ORGAN CULTURING; PROTEIN SYNTHESIS; RIBONUCLEIC ACIDS; TRANSCRIPTION (GENETICS)
Other Descriptors: GENE EXPRESSION REGULATION/DRUG EFFECTS/PHYSIOLOGY; NUCLEAR PROTEINS/GENETICS; PINEAL GLAND/PHYSIOLOGY; ADRENERGIC BETA-AGONISTS/PHARMACOLOGY; ADRENERGIC BETA-ANTAGONISTS/PHARMACOLOGY; ANIMALS; ARYLAMINE N-ACETYLTRANSFERASE/GENETICS; CIRCADIAN RHYTHM/PHYSIOLOGY; CYCLIC AMP/METABOLISM; CYCLIC GMP/METABOLISM; FORSKOLIN/PHARMACOLOGY; IN VITRO; MAP KINASE SIGNALING SYSTEM/PHYSIOLOGY; MALE; NOREPINEPHRINE/PHARMACOLOGY; ORGAN CULTURE; PROTEIN SYNTHESIS INHIBITORS/PHARMACOLOGY; RNA, MESSENGER/ANALYSIS; RATS; RATS, WISTAR; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; SYMPATHOMIMETICS/PHARMACOLOGY; TRANS-ACTIVATORS/METABOLISM; TRANSCRIPTION FACTORS/METABOLISM; TRANSCRIPTION, GENETIC/PHYSIOLOGY; NASA DISCIPLINE SPACE HUMAN FACTORS; NON-NASA CENTER
Availability Source: Other Sources
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