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Expression and affinity purification of recombinant proteins from plantsWith recent advances in plant biotechnology, transgenic plants have been targeted as an inexpensive means for the mass production of proteins for biopharmaceutical and industrial uses. However, the current plant purification techniques lack a generally applicable, economic, large-scale strategy. In this study, we demonstrate the purification of a model protein, beta-glucuronidase (GUS), by employing the protein calmodulin (CaM) as an affinity tag. In the proposed system, CaM is fused to GUS. In the presence of calcium, the calmodulin fusion protein binds specifically to a phenothiazine-modified surface of an affinity column. When calcium is removed with a complexing agent, e.g., EDTA, calmodulin undergoes a conformational change allowing the dissociation of the calmodulin-phenothiazine complex and, therefore, permitting the elution of the GUS-CaM fusion protein. The advantages of this approach are the fast, efficient, and economical isolation of the target protein under mild elution conditions, thus preserving the activity of the target protein. Two types of transformation methods were used in this study, namely, the Agrobacterium-mediated system and the viral-vector-mediated transformation system. Copyright 2002 Elsevier Science (USA).
Document ID
20040088264
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Desai, Urvee A.
(University of Kentucky Lexington, KY 40506, United States)
Sur, Gargi
Daunert, Sylvia
Babbitt, Ruth
Li, Qingshun
Date Acquired
August 21, 2013
Publication Date
June 1, 2002
Publication Information
Publication: Protein expression and purification
Volume: 25
Issue: 1
ISSN: 1046-5928
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Life Sciences Technologies
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