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Production of minimally disturbed synchronous cultures of hematopoietic cellsA method is describedforproducing sizable quantities of synchronously dividing, minimally disturbed mammalian cells. Cultures were grown immobilized on surfaces such that cell division within the population resulted in the continuous release of synchronous newborn cells. As judged by the quality and duration of synchronous growth, cell size distributions, and DNA compositions, newborn mouse L1210 cells grew with a very high level of synchrony without overt evidence of growth disturbances. The technology should be applicable to a variety of hematopoietic cells, as evidenced by similar results with human MOLT-4 and U937 cell lines.
Document ID
20040088319
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Thornton, Maureen
(Florida Institute of Technology Melbourne, FL 32901-6975, United States)
Eward, Kathryn Leigh
Helmstetter, Charles E.
Edward, K. L.
Date Acquired
August 21, 2013
Publication Date
May 1, 2002
Publication Information
Publication: BioTechniques
Volume: 32
Issue: 5
ISSN: 0736-6205
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: AG15187
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology
NASA Program Fundamental Space Biology

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