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High performance liquid chromatographic assay for the quantitation of total glutathione in plasmaA simple and widely used homocysteine HPLC procedure was applied for the HPLC identification and quantitation of glutathione in plasma. The method, which utilizes SBDF as a derivatizing agent utilizes only 50 microl of sample volume. Linear quantitative response curve was generated for glutathione over a concentration range of 0.3125-62.50 micromol/l. Linear regression analysis of the standard curve exhibited correlation coefficient of 0.999. Limit of detection (LOD) and limit of quantitation (LOQ) values were 5.0 and 15 pmol, respectively. Glutathione recovery using this method was nearly complete (above 96%). Intra-assay and inter-assay precision studies reflected a high level of reliability and reproducibility of the method. The applicability of the method for the quantitation of glutathione was demonstrated successfully using human and rat plasma samples.
Document ID
20040088326
Document Type
Reprint (Version printed in journal)
Authors
Abukhalaf, Imad K. (Morehouse School of Medicine Atlanta, GA 30310, United States)
Silvestrov, Natalia A.
Menter, Julian M.
von Deutsch, Daniel A.
Bayorh, Mohamed A.
Socci, Robin R.
Ganafa, Agaba A.
Date Acquired
August 21, 2013
Publication Date
May 15, 2002
Publication Information
Publication: Journal of pharmaceutical and biomedical analysis
Volume: 28
Issue: 4-Mar
ISSN: 0731-7085
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 2P20RR11104-06
CONTRACT_GRANT: S06GM08248-12
CONTRACT_GRANT: S06GM08248-06
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cardiopulmonary