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Remote sensing of gene expression in Planta: transgenic plants as monitors of exogenous stress perception in extraterrestrial environmentsTransgenic arabidopsis plants containing the alcohol dehydrogenase (Adh) gene promoter fused to the green fluorescent protein (GFP) reporter gene were developed as biological sensors for monitoring physiological responses to unique environments. Plants were monitored in vivo during exposure to hypoxia, high salt, cold, and abcissic acid in experiments designed to characterize the utility and responses of the Adh/GFP biosensors. Plants in the presence of environmental stimuli that induced the Adh promoter responded by expressing GFP, which in turn generated a detectable fluorescent signal. The GFP signal degraded when the inducing stimulus was removed. Digital imaging of the Adh/GFP plants exposed to each of the exogenous stresses demonstrated that the stress-induced gene expression could be followed in real time. The experimental results established the feasibility of using a digital monitoring system for collecting gene expression data in real time from Transgenic Arabidopsis Gene Expression System (TAGES) biosensor plants during space exploration experiments.
Document ID
20040088352
Document Type
Reprint (Version printed in journal)
Authors
Manak, Michael S. (University of Florida Gainesville, FL 32611-0690, United States)
Paul, Anna-Lisa
Sehnke, Paul C.
Ferl, Robert J.
Date Acquired
August 21, 2013
Publication Date
January 1, 2002
Publication Information
Publication: Life support & biosphere science : international journal of earth space
Volume: 8
Issue: 2
ISSN: 1069-9422
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAG10-291
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Plant Biology
Non-NASA Center